| 0 | - | cover | 1 | pencils: Dike Ruan; inks: Dike Ruan; colors: Alejandro Sánchez | Miles Morales, Spider-Man |
| 1 | - | promo (ad from the publisher) | 1 | letters: typeset | |
| 2 | - | credits, title page | 3 | letters: typeset | |
| 3 | 1 | cover reprint (on interior page) | 1 | pencils: Dike Ruan; inks: Dike Ruan; colors: Alejandro Sánchez | Miles Morales, Spider-Man |
| 4 | Trial by Spider: Part One | comic story | 30 | script: Cody Ziglar; pencils: Federico Vicentini; inks: Federico Vicentini; colors: Bryan Valenza; letters: VC's Cory Petit | Julia Gao, Bumbler, Mac Gargan, Ganke Lee, Jefferson Morales, Miles Morales, Rio Morales, Raneem Rashad, Scorpion, Spider-Man |
| 5 | - | blank page(s) | 1 | | |
| 6 | 2 | cover reprint (on interior page) | 1 | pencils: Dike Ruan; inks: Dike Ruan; colors: Alejandro Sánchez | Mac Gargan, Miles Morales, Scorpion, Spider-Man |
| 7 | Trial by Spider: Part Two | comic story | 20 | script: Cody Ziglar; pencils: Federico Vicentini; inks: Federico Vicentini; colors: Bryan Valenza; letters: VC's Cory Petit | Mac Gargan, Misty Knight, Ganke Lee, Miles Morales, Raneem Rashad, Scorpion, Spider-Man, Starling, Tiana Toomes |
| 8 | 3 | cover reprint (on interior page) | 1 | pencils: Dike Ruan; inks: Dike Ruan; colors: Alejandro Sánchez | Miles Morales, Rabble, Raneem Rashad, Spider-Man |
| 9 | Trial by Spider: Part Three | comic story | 20 | script: Cody Ziglar; pencils: Federico Vicentini; inks: Federico Vicentini; colors: Bryan Valenza; letters: VC's Cory Petit | Julia Gao, Mac Gargan, Misty Knight, Miles Morales, Rabble, Raneem Rashad, Scorpion, Spider-Man, Starling, Tiana Toomes |
| 10 | 4 | cover reprint (on interior page) | 1 | pencils: Dike Ruan; inks: Dike Ruan; colors: Alejandro Sánchez | Miles Morales, Rabble, Raneem Rashad, Spider-Man |
| 11 | Trial by Spider: Part Four | comic story | 20 | script: Cody Ziglar; pencils: Federico Vicentini; inks: Federico Vicentini; colors: Bryan Valenza; letters: VC's Cory Petit | Misty Knight, Ganke Lee, Miles Morales, Rabble, Raneem Rashad, Spider-Man, Starling, Tiana Toomes |
| 12 | - | blank page(s) | 1 | | |
| 13 | 5 | cover reprint (on interior page) | 1 | pencils: Dike Ruan; inks: Dike Ruan; colors: Alejandro Sánchez | Billie Morales, Jefferson Morales, Miles Morales, Rio Morales, Spider-Man |
| 14 | Trial by Spider: Part Five | comic story | 20 | script: Cody Ziglar; pencils: Federico Vicentini; inks: Federico Vicentini; colors: Bryan Valenza; letters: VC's Cory Petit | Julia Gao, Linda Carter, Mac Gargan, Misty Knight, Ganke Lee, Billie Morales, Jefferson Morales, Miles Morales, Rio Morales, Night Nurse, Rabble, Raneem Rashad, Scorpion, Spider-Man |
| 15 | #1 Variant | cover reprint (on interior page) | 1 | pencils: Federico Vicentini; inks: Federico Vicentini; colors: Bryan Valenza | |
| 16 | #1 Costume A Variant | cover reprint (on interior page) | 1 | pencils: Peach Momoko; inks: Peach Momoko; colors: Peach Momoko | |
| 17 | #1 Costume B Variant | cover reprint (on interior page) | 1 | pencils: Peach Momoko; inks: Peach Momoko; colors: Peach Momoko | |
| 18 | #1 Connecting Variant | cover reprint (on interior page) | 0.25 | pencils: Bengal; inks: Bengal; colors: Bengal | |
| 19 | #1 Graffiti Variant | cover reprint (on interior page) | 0.25 | pencils: Mike Del Mundo, Bacon; inks: Mike Del Mundo, Bacon; colors: Mike Del Mundo, Bacon | |
| 20 | #1 Cat Variant | cover reprint (on interior page) | 0.5 | pencils: Chrissie Zullo; inks: Chrissie Zullo; colors: Chrissie Zullo | |
| 21 | #1 Variant | cover reprint (on interior page) | 0.25 | pencils: Olivier Coipel; inks: Olivier Coipel; colors: Alejandro Sánchez | |
| 22 | #1 Variant | cover reprint (on interior page) | 0.25 | pencils: Taurin Clarke; inks: Taurin Clarke; colors: Taurin Clarke | |
| 23 | #2 Classic Homage Variant | cover reprint (on interior page) | 0.25 | pencils: Stephanie Hans; inks: Stephanie Hans; colors: Stephanie Hans | |
| 24 | #2 Design Variant | cover reprint (on interior page) | 0.25 | pencils: Federico Vicentini; inks: Federico Vicentini; colors: Federico Vicentini | |
| 25 | #2 Graffiti Variant | cover reprint (on interior page) | 0.25 | pencils: Nicoletta Baldari; inks: Nicoletta Baldari; colors: Nicoletta Baldari | |
| 26 | #2 Variant | cover reprint (on interior page) | 0.25 | pencils: JonBoy Meyers; inks: JonBoy Meyers; colors: JonBoy Meyers | |
| 27 | #3 Graffiti Variant | cover reprint (on interior page) | 0.25 | pencils: Benjamin Su; inks: Benjamin Su; colors: Benjamin Su | |
| 28 | #3 Variant | cover reprint (on interior page) | 0.25 | pencils: Ken Lashley; inks: Ken Lashley; colors: Juan Fernandez | |
| 29 | #4 Variant | cover reprint (on interior page) | 0.25 | pencils: Ryan Stegman; inks: JP Mayer; colors: Brad Anderson | |
| 30 | #4 Variant | cover reprint (on interior page) | 0.25 | pencils: Chris Bachalo; inks: Chris Bachalo; colors: Chris Bachalo | |
| 31 | #4 Timeless Variant | cover reprint (on interior page) | 0.25 | pencils: Alex Ross; inks: Alex Ross; colors: Alex Ross | |
| 32 | #4 Timeless Sketch Variant | cover reprint (on interior page) | 0.25 | pencils: Alex Ross | |
| 33 | #5 Timeless Variant | cover reprint (on interior page) | 0.25 | pencils: Alex Ross; inks: Alex Ross; colors: Alex Ross | |
| 34 | #5 Timeless Sketch Variant | cover reprint (on interior page) | 0.25 | pencils: Alex Ross | |
| 35 | #5 Variant | cover reprint (on interior page) | 0.5 | pencils: David Marquez; inks: David Marquez; colors: Alejandro Sánchez | |
| 36 | - | promo (ad from the publisher) | 1 | letters: typeset | |
| 37 | - | promo (ad from the publisher) | 1 | pencils: Dike Ruan; inks: Dike Ruan; colors: Alejandro Sánchez | Miles Morales, Rabble, Raneem Rashad, Spider-Man |