First appearance (advertisement) of Aunt Zelda - the earliest appearance on record is an advertisement, not a story.
First appearance (advertisement) of Scooby-Doo - the earliest appearance on record is an advertisement, not a story.
First appearance (advertisement) of Shaggy Rogers - the earliest appearance on record is an advertisement, not a story.
First appearance (advertisement) of Velma Dinkley - the earliest appearance on record is an advertisement, not a story.
First appearance (advertisement) of Fred Jones - the earliest appearance on record is an advertisement, not a story.
First appearance (advertisement) of Daphne Blake - the earliest appearance on record is an advertisement, not a story.
First appearance (advertisement) of Lee Rider - the earliest appearance on record is an advertisement, not a story.
First appearance (advertisement) of Sylvester Sneekly - the earliest appearance on record is an advertisement, not a story.
First appearance (advertisement) of Hooded Claw - the earliest appearance on record is an advertisement, not a story.
First appearance (advertisement) of Klunk - the earliest appearance on record is an advertisement, not a story.
First appearance (advertisement) of Zilly - the earliest appearance on record is an advertisement, not a story.
First appearance (advertisement) of Yankee Doodle Pigeon - the earliest appearance on record is an advertisement, not a story.
The evidence
Claims here come from the story records for this issue, listed under Sources at the bottom of the file. Character hubs with full evidence tables are coming to this site.