| 0 | - | cover | 1 | pencils: Bryan Hitch; inks: Paul Neary | Amazo, Bonny Hoffman First, Carmen Leno First, Ma'alefa'ak First, Malefic First, Mawzir, Neron, Vandal Savage |
| 1 | DCU Villains Secret Files and Origins | credits, title page | 1 | | Abra Kadabra; Luthor; Joker; Neron |
| 2 | Contents | credits, title page | 1 | letters: typeset | |
| 3 | The Evil That We Do | comic story | 22 | script: J. H. Williams, Dan Curtis Johnson; pencils: Eric Canete; inks: Shawn Martinbrough; colors: Digital Chameleon; letters: Kurt Hathaway | Vandal Savage; The Word; Body Doubles; Cheshire; Enchantress [June Moon]; Houngan; Pistolera; Tannarak; Vicious; The Wizard; Cameron Chase; Doctor Occult; Ibis; J'Onn J'Onzz; Madame Xanadu; Resurrection Man [Mitchell Shelley] |
| 4 | Vandal Savage | character profile | 1 | script: Scott Beatty; pencils: Val Semeiks; inks: Prentis Rollins; colors: Tom McCraw, Digital Chameleon | Vandal Savage; Cameo - Solaris |
| 5 | Lost Pages - Vandal Savage vs. Resurrection Man | comic story | 4 | script: Dan Abnett, Andy Lanning; pencils: Phil Winslade; inks: Phil Winslade; colors: Tom McCraw, Digital Chameleon; letters: Ellie de Ville | Vandal Savage; Resurrection Man [Mitchell Shelley]; Dr. Fate [Kent Nelson] (cameo); Flash [Jay Garrick] (cameo); Green Lantern [Alan Scott] (cameo); Hawkman [Carter Hall] (cameo); Sandman [Wesley Dodds] (cameo); Wonder Woman [Hippolyta] (cameo) |
| 6 | The Star Conqueror | character profile | 1 | script: Scott Beatty; pencils: Barry Kitson; inks: Barry Kitson; colors: Tom McCraw, Digital Chameleon | Starro; Aquaman (cameo); Batman [Bruce Wayne] (cameo); Flash [Wally West] (cameo); Green Lantern [Kyle Rayner] (cameo); J'Onn J'Onzz (cameo); Wonder Woman [Diana] (cameo) |
| 7 | Shiv | character profile | 1 | script: Geoff Johns; pencils: Lee Moder; inks: Dan Davis; colors: Tom McCraw, Digital Chameleon | Shiv |
| 8 | Body Doubles | character profile | 1 | script: Dan Abnett, Andy Lanning; pencils: Joe Phillips; inks: Jasen Rodriguez; colors: Tom McCraw, Digital Chameleon | Body Doubles |
| 9 | A Day in the Life of the Body Doubles | comic story | 2 | script: Dan Abnett, Andy Lanning; pencils: Joe Phillips; inks: Jasen Rodriguez; colors: Tom McCraw, Digital Chameleon; letters: Bill Oakley | Body Doubles |
| 10 | Neron | character profile | 1 | script: Scott Beatty; pencils: Derec Aucoin; inks: Rich Faber; colors: Tom McCraw, Digital Chameleon | Neron |
| 11 | Infernal Villains: Etrigan Meets Neron | comic story | 2 | script: Alan Grant; pencils: Ariel Olivetti; inks: Ariel Olivetti; colors: Tom McCraw, Digital Chameleon; letters: Bill Oakley | Demon; Neron; American Screem (cameo); Blaze (cameo); Circe (cameo); Dr. Alchemy [Albert Desmond] (cameo); Felix Faust (cameo); Gray Man (cameo); Klarion (cameo); Lord Satanus (cameo); Temptress (cameo) |
| 12 | The Mawzir | character profile | 1 | script: Scott Beatty; pencils: John McCrea; inks: John McCrea; colors: Tom McCraw, Digital Chameleon | The Mawzir |
| 13 | Mystical Menaces | character profile | 2 | script: Scott Beatty; pencils: Dusty Abell; inks: Dusty Abell; colors: Tom McCraw, Digital Chameleon | Azarathea; Diabola; Doctor 7; Enchantress [June Moon]; Faust; Gray Man; Houngan; Lord Ys; Qabala; Tannarak; Trance; The Wizard |
| 14 | The Ravens | character profile | 1 | script: Chuck Dixon; pencils: Jason Orfalas; inks: Marlo Alquiza; colors: Tom McCraw, Digital Chameleon | Cheshire; Pistolera; Vicious |
| 15 | The Ravens' Rap Sheet | comic story | 1 | script: Chuck Dixon; pencils: Jason Orfalas; inks: Marlo Alquiza; colors: Tom McCraw, Digital Chameleon | Cheshire; Pistolera; Termina; Vicious |
| 16 | Amazo | character profile | 1 | script: Scott Beatty; pencils: Rags Morales; inks: Ray Kryssing; colors: Tom McCraw, Digital Chameleon | Amazo |
| 17 | Malefic | comic story | 1 | script: John Ostrander; pencils: Tom Mandrake; inks: Tom Mandrake; colors: Tom McCraw, Digital Chameleon; letters: Ken Lopez | Malefic |
| 18 | Malefic | character profile | 1 | script: Scott Beatty; pencils: Tom Mandrake; inks: Tom Mandrake; colors: Tom McCraw, Digital Chameleon | Malefic |
| 19 | Buzzword & Dolmen | character profile | 1 | script: Alan Grant; pencils: Norm Breyfogle; inks: Norm Breyfogle; colors: Tom McCraw, Digital Chameleon | Buzzword; Dolmen |
| 20 | "The Slab" Map | comic story | 1 | script: Eliot Brown; pencils: Eliot Brown; inks: Eliot Brown; colors: Digital Chameleon | |
| 21 | Mr. Mind | comic story | 1 | script: Scott Beatty; pencils: Peter Krause; inks: Jerry Ordway; colors: Tom McCraw, Digital Chameleon | Mister Mind |
| 22 | Mr. Mind Maze | activity | 1 | script: Erik Doescher; pencils: Erik Doescher; inks: Charlie La Greca; colors: Digital Chameleon | |
| 23 | Solaris | character profile | 1 | script: Scott Beatty; pencils: Anthony Williams; inks: Andy Lanning; colors: Tom McCraw, Digital Chameleon | Solaris |
| 24 | Solaris Schematic | comic story | 1 | script: Eliot Brown; pencils: Eliot Brown; inks: Eliot Brown; colors: Digital Chameleon | Solaris |
| 25 | Vandal Savage's Trophy Room | comic story | 2 | script: Dan Abnett, Andy Lanning; pencils: Jason Orfalas; inks: Marlo Alquiza; colors: Digital Chameleon; letters: Bill Oakley | Vandal Savage |
| 26 | DCU Villains Timeline | comic story | 2 | script: Scott Beatty | |
| 27 | The Word | character profile | 1 | script: D. Curtis Johnson; pencils: J. H. Williams III; inks: Mick Gray; colors: Tom McCraw, Digital Chameleon | The Word |