| 0 | - | cover | 1 | pencils: Jerry Robinson; inks: Jerry Robinson | Batman, Dick Grayson, Robin, Bruce Wayne |
| 1 | Editorial Advisory Board | promo (ad from the publisher) | 0.33 | letters: typeset | |
| 2 | - | in-house column | 0.67 | script: Josette Frank | |
| 3 | The Batman Plays a Lone Hand! | comic story | 13 | script: Don C. Cameron; pencils: Bob Kane; inks: Jerry Robinson, George Roussos; letters: George Roussos | Batman [Bruce Wayne]; Robin [Dick Grayson]; Commissioner James Gordon; The Penguin [Oswald Cobblepot] (villain, cameo); The Joker (villain, cameo); The Thumb (villain, introduction); Thumb's henchmen (villains) |
| 4 | Round-House Willie | comic story | 2 | script: Bob Naylor; pencils: Bob Naylor; inks: Bob Naylor; letters: Bob Naylor | Round-House Willie; Mr. Nuggins; Mr. Snithers; Mr. Crimp |
| 5 | Laffs | cartoon | 0.5 | script: Henry Boltinoff; pencils: Henry Boltinoff; inks: Henry Boltinoff; letters: Henry Boltinoff | Willy |
| 6 | Double Trouble for Batman and Robin!! | promo (ad from the publisher) | 0.5 | | |
| 7 | Comedy of Tears! | comic story | 13 | script: Jack Schiff; pencils: Bob Kane; inks: Jerry Robinson, George Roussos; letters: George Roussos | Batman, Joe DiMaggio, Commissioner James Gordon, Dick Grayson, Joker, Robin, Jerry Siegel, Bruce Wayne |
| 8 | We've Pepped 'Em Up... | promo (ad from the publisher) | 1 | | |
| 9 | Tubby | comic story | 1 | pencils: George Storm; inks: George Storm | Tubby |
| 10 | The Story of the Seventeen Stones! | comic story | 13 | script: Bill Finger; pencils: Jack Burnley; inks: Jack Burnley, Ray Burnley; letters: Betty Bentley | Batman [Bruce Wayne]; Robin [Dick Grayson]; Commissioner James Gordon; Mason (reformed villain, architect, introduction); Brenner (reformed villain, diamond cutter); Parks (reformed villain); Rocky Grimes (villain, introduction, death); Lefty Slade (villain, introduction, death); "Fin" Gonzy (villai |
| 11 | - | comic story | 0.22 | script: Henry Boltinoff; pencils: Henry Boltinoff; inks: Henry Boltinoff; letters: Henry Boltinoff | Clancy the Cop |
| 13 | DC Comics Give You What You Like! | promo (ad from the publisher) | 0.67 | | |
| 14 | Sacrifice | text story | 2 | script: Eric Carter | Lane Thomas; Little Aussie; Marco Evans |
| 15 | The Flying Jeeps | comic story | 2 | pencils: George Storm; inks: George Storm | |
| 16 | - | comic story | 0.5 | script: Henry Boltinoff; pencils: Henry Boltinoff; inks: Henry Boltinoff; letters: Henry Boltinoff | Silly Willy |
| 17 | Biggest and Best! | promo (ad from the publisher) | 0.5 | | |
| 18 | - | comic story | 1 | script: Henry Boltinoff; pencils: Henry Boltinoff; inks: Henry Boltinoff; letters: Henry Boltinoff | Jerry the Jitterbug |
| 19 | Destination Unknown! | comic story | 13 | script: Don C. Cameron; pencils: Bob Kane; inks: Jerry Robinson, George Roussos; letters: George Roussos | Batman [Bruce Wayne]; Robin [Dick Grayson]; Detective Guffey; Commissioner James Gordon; Clyde Clayborn (collector of oddities); Miss Hibbs (Clayborn's Secretary); Mr. Fortesque (man in an iron lung, actually a wax dummy); John Keyes (innocent prisoner); Ken Thorne (President of the railroad); Trigg |
| 20 | The Big Eight! | promo (ad from the publisher) | 1 | | |