| 0 | - | cover | 1 | pencils: Joe Staton; inks: Bob Hall; colors: Rosario Peña | Tommy Troy; the Fly; the Spider |
| 1 | - | table of contents | 2 | script: Paul Castiglia | |
| 2 | Fly on the Wall | foreword, introduction, preface, afterword | 2 | script: Joe Simon | |
| 3 | The Hide-Out | comic story | 2 | script: Joe Simon, Jack Kirby; pencils: Jack Kirby; letters: Sam Rosen | The Fly (first appearance); Mike |
| 4 | The Boy Sentinels' Assignment: Find Tommy Troy | comic story | 2 | script: Joe Simon; pencils: Joe Simon; inks: Joe Simon; letters: Howard Ferguson | The Fly [Tommy Troy]; the Boy Sentinels |
| 5 | Adventures of the Fly #1 | cover reprint (on interior page) | 1 | pencils: Jack Kirby; inks: Joe Simon | The Fly [Tommy Troy]; the Spider [Spider Spry] |
| 6 | The Strange New World of the Fly | comic story | 8 | script: Joe Simon, Jack Kirby; pencils: Jack Kirby; inks: Joe Simon, Jack Kirby; letters: Sam Rosen | The Fly [Tommy Troy] (origin); Billy; Mr. Creacher; McCoy; Ben March; Abigail March; Turan |
| 7 | The Fly Discovers His Buzz Gun | comic story | 5 | script: Joe Simon, Jack Kirby; pencils: Jack Kirby; inks: Joe Simon; letters: Sam Rosen | The Fly [Tommy Troy]; Ezra March [Ben March]; Abigail March; Dolly Lake |
| 8 | Come into My Parlor | comic story | 6 | script: Joe Simon, Jack Kirby; pencils: Jack Kirby; inks: Joe Simon, Jack Kirby; letters: Sam Rosen | The Fly [Tommy Troy]; Spider Spry [the Spider] (first appearance); Bruiser |
| 9 | Magic Eyes | comic story | 4 | script: Joe Simon; pencils: George Tuska; inks: Bill Draut; letters: Sam Rosen | The Fly [Tommy Troy]; Dolly Lake; Mr. Lighthouse; Glen Glim |
| 10 | Adventures of the Fly #2 | cover reprint (on interior page) | 1 | pencils: Joe Simon; inks: Joe Simon | The Fly [Tommy Troy]; Tim O'Casey |
| 11 | Tim O'Casey's Wrecking Crew! | comic story | 6 | script: Joe Simon; pencils: Dick Ayers, Joe Simon; inks: Paul Reinman, Joe Simon; letters: Howard Ferguson | The Fly [Tommy Troy]; Tim O'Casey; Bertrand; Alec Drews; Finn MacCool; Ben March |
| 12 | One of Our Skyscrapers is Missing! | comic story | 4 | script: Joe Simon; pencils: Al Williamson; inks: Angelo Torres; letters: Sam Rosen | The Fly [Tommy Troy]; Dolly Lake |
| 13 | Marco's Eyes | comic story | 7 | script: Joe Simon; pencils: Jack Kirby; inks: Joe Simon, Jack Kirby; letters: Howard Ferguson | The Fly [Tommy Troy]; Mister Marco; Dolly Lake; Freddy Freckle; Slats Dillon; J.B. Bascombe |
| 14 | The Master of Junk-Ri-La! | comic story | 6 | script: Joe Simon, Jack Kirby; pencils: Jack Kirby; inks: Joe Simon, Paul Reinman; letters: Howard Ferguson | The Fly [Tommy Troy]; Ben March; Hans Yunkmann [The Junkman] |
| 15 | Adventures of the Fly #3 | cover reprint (on interior page) | 1 | pencils: Joe Simon; inks: Joe Simon | The Fly [Tommy Troy] |
| 16 | The Overblown Marshall of Spire City! | comic story | 6 | script: Joe Simon; pencils: Jack Davis, Joe Simon; inks: Joe Simon, Jack Davis; letters: Howard Ferguson | The Fly [Tommy Troy]; Marshal Bruno; Commissioner Daley; Dolly Lake |
| 17 | How an Orphan Boy Became the Fearsome Fly | comic story | 2 | script: Joe Simon; pencils: Joe Simon; inks: Joe Simon | The Fly [Tommy Troy]; Mr. Creacher; Bruiser; Ben March; Abigail March; Turan |
| 18 | The House of Solid Nothing | comic story | 5 | script: Joe Simon; pencils: Bob Powell; inks: Bob Powell | The Fly [Tommy Troy]; Ezra Clump; Mr. Clinton; Dolly Lake |
| 19 | Adventures of the Fly #4 | cover reprint (on interior page) | 1 | pencils: Joe Simon; inks: Joe Simon | The Fly [Tommy Troy]; Mugsy Malone |
| 20 | Duped by the Dazzler! | comic story | 8 | script: Joe Simon; pencils: Ted Galindo, Joe Simon; inks: Chic Stone, Joe Simon | The Fly [Tommy Troy]; Daniel Domino [the Dazzler]; Ben March; Joshua Flint; Abigail March |
| 21 | Muggsy's Masterpieces! | comic story | 8 | script: Joe Simon; pencils: Joe Simon, Neal Adams; inks: Joe Simon, Neal Adams; letters: Howard Ferguson | The Fly [Tommy Troy]; Dolly Lake; Muggsy Malone; Ben March; Spike |
| 22 | Slide Rule's Challenge! | comic story | 8 | script: Joe Simon; pencils: Bob Powell; inks: Bob Powell | The Fly [Tommy Troy]; Slide-Rule; Dolly Lake; Ben March; Abigail March |